Natural Sciences
Mak Scientists Unveil New Method of Measuring Stress Levels in Plants
Just like animals and human beings, plants too can be stressed. Whereas one may easily detect stress in animals and humans basing on their behavior, plant “behavior” is not easy to interpret.
Why should we be concerned about stress in plants?
Plants under stress cannot reproduce or grow well because they have to divert much of their physiological energy to fighting stress. If the plants are critical food crops, this will lead to perpetual food shortages because their energy resources are spent fighting stress.
New study on measuring stress in plants
A new study titled “Sandwich Enzyme-Linked Immunosorbent Assay for Quantification of Callose” and led by a team of scientists at the Department of Plant Sciences, Microbiology and Biotechnology at Makerere University in collaboration with the University of California, Davis, USA, shows how stress in plants can be measured. According to Prof. Arthur Tugume, the lead scientist of this study and expert in plant diseases, when plants are stressed, they produce numerous molecules within their cells mostly as means of adapting to stress or avoiding the impact of stress. “For example, plants produce reactive oxygen species like hydrogen peroxide, superoxide ions, and hydroxyl ions. These reactive oxygen species are produced rapidly and act as rapid messengers in the plant tissues to activate additional responses spreading over the entire plant body. This helps the plant to withstand or avoid the impact of stress”.
Dr. Ssenku E. Jamilu, a plant physiology expert on the team explains that in plants, stress can be induced by many factors such as pollution in the soil and atmosphere, high soil salinity (salt stress), excessive lack of water (drought), extreme temperatures, lack of oxygen (anoxia), excess radiation, mechanical injury by wounding or by pests that feed on the plants, and infestation by disease-causing microorganisms (pathogens). “Such factors are worsened by climate change, implying the importance of measuring their impact on individual plants to guide plant breeding programmes to ensure sustainable food production in the face of climate change”.
The research indicates that reactive oxygen species set in motion additional processes to ensure limited impact of stress on the plant. For example, a unique carbohydrate, named “callose” starts to accumulate in large quantities within plant cells as a means of fortifying plant cells. Callose differs from the other usual plant carbohydrates such as starch or cellulose because of the way its structures are formed. Also, the production of callose gets increased during stress. Callose acts as a road-block to any pathogen such as bacteria by limiting bacterial movement that would otherwise ease attack on other tissues or cells.
“If we can artificially induce the stress on the plant, and then measure the amount of reactive oxygen species or callose or any other responses in the plant, we should be able to directly and quantitatively measure the stress,” Prof. Tugume explains.
He however notes that callose participates in many other normal developmental processes of plants and for that reason, there is always some “housekeeping” callose in the plant tissues even without stress. “This means that one must be able to accurately and quantitatively distinguish between ‘stress-induced’ and normal ‘housekeeping’ callose”.
In this study, the researchers used young (2.5-months old) banana plantlets that had been generated from tissue culture at Kawanda Agricultural Research Institute. They then infected the plantlets with a bacterium called Xanthomonas campestris pv. musacearum. This bacterium induces so much stress on the banana plants resulting into a destructive disease known as banana xanthomonas wilt (BXW), the most destructive disease of bananas in East and Central Africa.
The banana leaves, pseudostems and corms were analyzed for callose and compared with the control plants that had been inoculated with water instead of bacteria. The analysis was done using a method called S-ELISA (Sandwich Enzyme-linked immunosorbent Assay), that was designed by scientists in this study. This method is specific to callose.
Callose-specific primary antibodies were used to coat the wells of 96-well microplates followed by incubation with callose extracts from tissues of stressed plants. Additional incubation with same primary callose-specific antibodies was done, and finally, incubation with a secondary antibody that is conjugated to an enzyme. When a substrate is supplied to the enzyme, colour changes were monitored. The presence of callose (and thus stress on the plants) was assessed quantitatively by measuring the intensity of color change resulting from enzymatic action on the substrate. This method proved effective in comparison to the gold standard method of detecting and quantifying aniline blue stained callose using fluorescence microscopy.
This research was part of the PhD studies for Mr. Abubakar S. Mustafa at Makerere University and University of California, Davis. According to Mr. Mustafa, his method is new and can be applied to any plant that is subjected to any stress because generally, plants produce callose in response to stress.” Mr. Mustafa further states that the method is convenient because, if necessary, samples can be processed between a few hours to one year (or even longer), which allows exchange of materials between laboratories and countries when needed, something that was not possible before. The S-ELISA method used 96-well plates which allows high throughout studies, hence, hundreds of samples can be analyzed simultaneously within a few days.
This study has been published as a Protocol by Multidisciplinary Publishing Institute (MDPI) in an open access journal, “Methods and Protocols”, in the section: Biochemical and Chemical Analysis & Synthesis and is freely accessible on https://www.mdpi.com/2409-9279/5/4/54/htm.
The research was funded by the Bill and Melinda Gates Foundation through the National Agricultural Research Organization (NARO), Uganda. The project had partners including the International Institute of Tropical Agriculture, the Alliance for Bioversity International and International Centre for Tropical Agriculture (CIAT) and CABI.
For more details, contact;
- Prof. Arthur Tugume, Lead Scientist of the study, College of Natural Sciences, Makerere University, Email: arthur.tugume@mak.ac.ug, Tel: +256772514841
- Mr. Abubakar S. Mustafa, Co-Author and PhD student on the study, Email: mustafa.abubakar.sadik@gmail.com, Tel: +256702813233
- Hasifa Kabejja, Principal Communication Officer, College of Natural Sciences, Makerere University, Email: pr.cns@mak.ac.ug, Tel: +256774904211
Natural Sciences
Change of Guard: Prof. Michael Owor Hands Over Office of Dean, School of Physical Sciences to Prof. David Ssevviiri
Prof. Michael Owor has officially handed over the office of Dean, School of Physical Sciences to Prof. David Ssevviiri. Prof. Owor has served as Dean since 1st February 2022. Following his appointment by the University Council, Prof. Ssevviiri will serve as Dean for the next four years, effective 6th May 2026.
Handover Ceremony
Held on 28th May 2026 in the office of the Dean, the handover ceremony was overseen by Makerere University Chief of Internal Audit, Mr. Patrick Akonyet. It was attended by the Deputy Principal of the College of Natural Sciences (CoNAS), Prof. Juma Kasozi; the Head of the Department of Chemistry, Dr Emmanuel Tebandeke; the representative of the Head of the Department of Physics, Dr. Nicholas Kwarikunda; the representative of the Head of the Department of Geology and Petroleum Studies, Dr Denis Mutebi; the College Human Resource Officer, Ms. Kevin Nabiryo Mikwano; and the College and School Registrars.
Remarks from the College leadership
On behalf of the College leadership, Prof. Juma Kasozi commended Prof. Owor for his dedicated service and leadership throughout his tenure and congratulated Prof. Ssevviiri upon his appointment, wishing him success in his new role.
Achievements and remarks by the outgoing Dean
During his tenure, Prof. Owor chaired the School Academic Board and several key committees, including the Establishment and Appointments Committee, the Examination Malpractices Committee, and the Irregularities and Appeals Committee. He also presided over MSc viva voce examinations and PhD public defenses. Under his leadership, he ensured the timely submission of results, eliminated academic backlogs, and strengthened the coordination of graduate examinations, while also supporting the development and review of academic programmes. He has also served as the Patron of the CoNAS Students’ Guild.
In his remarks, Prof. Owor expressed gratitude to the University Council and Management for the opportunity accorded to him to serve as Dean. He also thanked the College leadership and staff for their support and reaffirmed his commitment to continue serving the University.
“I want to thank the University Management through the Chief Human Resource Officer for appointing me as dean, School of Physical Sciences for the last four years. I pledge my total support to the incoming Dean of the School. I also pledge to continue offering my services to the University through my mother Department, Geology and Petroleum Studies.”
Remarks from the Directorate of Human Resources
Representing the Directorate of Human Resources (DHR), Ms. Kevin Nabiryo Mikwano commended Prof. Owor for his leadership and contributions during his tenure.
“You have steered the school through curriculum reforms, staff development, student growth, and your commitment to both staff and students has not gone unnoticed. The foundation you have laid will continue to support the school’s progress. Thank you for your partnership with the Directorate of Human Resources in ensuring staff welfare, recruitment, and performance processes were handled with integrity and care.”
She also congratulated Prof. Ssevviiri on his appointment, expressing confidence in his leadership and vision. She reaffirmed the Directorate’s readiness to support him in team building, talent management, and fostering a positive working environment for academic, administrative, and support staff. She further encouraged staff to maintain strong collaboration with the incoming Dean, emphasizing: “The success of the school depends on how well we work together. At the DHR, our commitment is to ensure that policies, processes, and people management practices support the school’s academic mission. May this handover mark the beginning of another strong chapter for the School of Physical Sciences.”
Guidance on the officially recognized Government handover procedure
The Chief of Internal Audit, Mr. Patrick Akonyet, commended both the outgoing and incoming Deans and emphasized adherence to the Government of Uganda handover and taking of office procedures. He noted that strict compliance with established guidelines is essential for accountability, transparency, institutional continuity, and smooth transition of responsibilities.
Remarks by the incoming Dean
In his remarks, Prof. David Ssevviiri thanked Prof. Owor for his service and appreciated the University Management for his appointment. He emphasized the importance of the School of Physical Sciences in advancing science and innovation. “We have a responsibility of nurturing our students into innovative problem solvers. The future of Science depends on how well we prepare our students. Building on the successes of my predecessors, and working collaboratively through our Departments, we can further contribute to solving real world challenges.
Profile of Prof. David Ssevviiri
Prof. David Ssevviiri is a Ugandan mathematician and academic leader serving as an Associate Professor of Mathematics at Makerere University, College of Natural Sciences. He is widely recognized for his work in pure mathematics, particularly in algebra, module theory, ring theory, algebraic geometry, and local cohomology.
He holds a Bachelor of Science with Education in Mathematics and Chemistry from Makerere University, as well as a Master of Science in Mathematics from the same institution. He also obtained an additional MSc and a PhD in Mathematics from Nelson Mandela Metropolitan University (now Nelson Mandela University), South Africa. His doctoral research, A Contribution to the Theory of Prime Modules, focused on advanced module theory and algebra.
Prof. Ssevviiri joined Makerere University in 2011 as an Assistant Lecturer and rose to the rank of Associate Professor in 2022. He previously served as Head of the Department of Mathematics from 2015 to 2020, where he contributed to strengthening teaching, research collaboration, and postgraduate training.
His research interests include primeness and nilpotency in ring and module theory, torsion theory, radical theory, homological algebra, and the interaction between algebra and algebraic geometry. He has authored and co-authored several peer-reviewed publications in international journals, contributing significantly to advancements in abstract algebra.
Beyond teaching and research, he has contributed to mathematics development in East Africa through student supervision, external examining, and coordination of regional academic programs. He has also participated in initiatives aimed at promoting mathematics education and encouraging young people to pursue careers in science and technology.
Pictorial of the ceremony: https://drive.google.com/drive/folders/1pmWX5H46UHbFkTT-I4OIJwFbXIwuO6e1?usp=sharing
A team of researchers from Makerere University has presented findings of a study on the persistent poor performance in Biology in national examinations to senior officials at the Ministry of Education and Sports.
The findings were shared during a dissemination meeting attended by the Ministry’s top management, led by Permanent Secretary Kedrace Turyagenda.
The study, commissioned by the Ministry in 2023, was conducted by researchers from the College of Natural Sciences (CoNAS) and the College of Education and External Studies (CEES). It was spearheaded by Prof. Fredrick Muyodi as Principal Investigator, alongside Assoc. Prof. Josephine Esaete (Co-Principal Investigator) and Prof. Arthur Tugume among others.
Titled “Examining the Factors Accounting for Poor Academic Performance in Biology in Uganda’s National Examinations,” the study covered all regions of the country, engaging more than 5,200 respondents across 100 districts through surveys, interviews, and observations.
Key findings
The research identified limited field-based learning and poor digital access as the most critical and consistent challenges affecting both Ordinary and Advanced Level students across all regions.
At Advanced Level, the lack of project-based learning was also cited as a significant constraint. Learners reported limited engagement with Biology beyond the classroom, a factor that weakens conceptual understanding and application in examinations.
The study further highlighted the role of community attitudes and perceptions in shaping student performance in the subject.
Recommendations
The researchers recommended increased investment in field-based learning, including field trips and project work, as well as expansion of digital infrastructure to improve access to learning resources.
Other proposed measures include strengthening school laboratory facilities, enhancing teacher capacity through continuous professional development, promoting student engagement through academic clubs and mentorship programmes, and fostering stronger family and community support for science education.
Next steps
The research team is seeking funding to pilot targeted interventions, particularly in regions most affected by the challenges identified, and to develop a monitoring and evaluation framework to guide implementation.
The findings are expected to inform policy and interventions aimed at improving Biology performance and strengthening science education in Uganda.
By Dr. Alice Nabatanzi
At the Department of Plant Sciences, Microbiology, and Biotechnology (PMB) in the College of Natural Sciences, we are moving beyond the classroom to bring science to life. We are proud to showcase our innovative work with the Biotechnology Class of 2026. Our Product Development Centre has transitioned from theoretical research to practical, market-ready innovations. By applying advanced scientific principles, our students are developing tangible solutions that address everyday needs in nutrition, health, and industry.
Below are the latest products developed by our talented innovators:
- Nutrition & Food Technology: Vitamin A-rich bread, banana vinegar, food syrups, and fortified bio-snacks.
- Clinical Hygiene: Perfumes, Deodorants, Antibacterial gels, herbal toothpaste, shaving cream, and herbal mouthwash.
- Natural Wellness: Natural formulas for gut health, pain relief, cough lozenges, and nasal decongestants.
- Advanced Cosmeceuticals: Turmeric SunGuard patches, skin serums, toners, and anti-dandruff shampoo.
- Home & Industrial Solutions: Shoe deodorizing sprays, surface cleaners, and scented hygiene products.
- Agri-Tech: Natural bioindicator kits designed to monitor and improve soil health.
Through these initiatives, PMB continues to lead the way in re-engineering science for the benefit of our community and the world.
Meet the Biotechnology Class of 2026. While everyone else is still stuck in textbooks writing about “how things might work,” we’re in the lab making sure they actually do. We’ve officially moved past the days of just talking about science and stepped into the era of real-world industry. From our Product Development Centre to the global market, we are setting a new standard for Biotechnology at CoNAS. We aren’t just graduating with degrees; we’re graduating with the future in our hands.
What’s Next: We’re Just Getting Started
This is only the beginning. We’re already working on the next wave of products, from eco-friendly bio-packaging and better probiotics to smart-crop tech that will change how we eat, heal, and live. We aren’t just waiting for the future to happen we’re in the lab building it right now.
Dr. Alice Nabatanzi is a Senior Lecturer of Phytomedicine and Nutraceuticals at the College of Natural Sciences (CoNAS), Makerere University
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