Natural Sciences
Mak Scientists Unveil New Method of Measuring Stress Levels in Plants
Just like animals and human beings, plants too can be stressed. Whereas one may easily detect stress in animals and humans basing on their behavior, plant “behavior” is not easy to interpret.
Why should we be concerned about stress in plants?
Plants under stress cannot reproduce or grow well because they have to divert much of their physiological energy to fighting stress. If the plants are critical food crops, this will lead to perpetual food shortages because their energy resources are spent fighting stress.
New study on measuring stress in plants
A new study titled “Sandwich Enzyme-Linked Immunosorbent Assay for Quantification of Callose” and led by a team of scientists at the Department of Plant Sciences, Microbiology and Biotechnology at Makerere University in collaboration with the University of California, Davis, USA, shows how stress in plants can be measured. According to Prof. Arthur Tugume, the lead scientist of this study and expert in plant diseases, when plants are stressed, they produce numerous molecules within their cells mostly as means of adapting to stress or avoiding the impact of stress. “For example, plants produce reactive oxygen species like hydrogen peroxide, superoxide ions, and hydroxyl ions. These reactive oxygen species are produced rapidly and act as rapid messengers in the plant tissues to activate additional responses spreading over the entire plant body. This helps the plant to withstand or avoid the impact of stress”.
Dr. Ssenku E. Jamilu, a plant physiology expert on the team explains that in plants, stress can be induced by many factors such as pollution in the soil and atmosphere, high soil salinity (salt stress), excessive lack of water (drought), extreme temperatures, lack of oxygen (anoxia), excess radiation, mechanical injury by wounding or by pests that feed on the plants, and infestation by disease-causing microorganisms (pathogens). “Such factors are worsened by climate change, implying the importance of measuring their impact on individual plants to guide plant breeding programmes to ensure sustainable food production in the face of climate change”.
The research indicates that reactive oxygen species set in motion additional processes to ensure limited impact of stress on the plant. For example, a unique carbohydrate, named “callose” starts to accumulate in large quantities within plant cells as a means of fortifying plant cells. Callose differs from the other usual plant carbohydrates such as starch or cellulose because of the way its structures are formed. Also, the production of callose gets increased during stress. Callose acts as a road-block to any pathogen such as bacteria by limiting bacterial movement that would otherwise ease attack on other tissues or cells.
“If we can artificially induce the stress on the plant, and then measure the amount of reactive oxygen species or callose or any other responses in the plant, we should be able to directly and quantitatively measure the stress,” Prof. Tugume explains.
He however notes that callose participates in many other normal developmental processes of plants and for that reason, there is always some “housekeeping” callose in the plant tissues even without stress. “This means that one must be able to accurately and quantitatively distinguish between ‘stress-induced’ and normal ‘housekeeping’ callose”.
In this study, the researchers used young (2.5-months old) banana plantlets that had been generated from tissue culture at Kawanda Agricultural Research Institute. They then infected the plantlets with a bacterium called Xanthomonas campestris pv. musacearum. This bacterium induces so much stress on the banana plants resulting into a destructive disease known as banana xanthomonas wilt (BXW), the most destructive disease of bananas in East and Central Africa.
The banana leaves, pseudostems and corms were analyzed for callose and compared with the control plants that had been inoculated with water instead of bacteria. The analysis was done using a method called S-ELISA (Sandwich Enzyme-linked immunosorbent Assay), that was designed by scientists in this study. This method is specific to callose.
Callose-specific primary antibodies were used to coat the wells of 96-well microplates followed by incubation with callose extracts from tissues of stressed plants. Additional incubation with same primary callose-specific antibodies was done, and finally, incubation with a secondary antibody that is conjugated to an enzyme. When a substrate is supplied to the enzyme, colour changes were monitored. The presence of callose (and thus stress on the plants) was assessed quantitatively by measuring the intensity of color change resulting from enzymatic action on the substrate. This method proved effective in comparison to the gold standard method of detecting and quantifying aniline blue stained callose using fluorescence microscopy.
This research was part of the PhD studies for Mr. Abubakar S. Mustafa at Makerere University and University of California, Davis. According to Mr. Mustafa, his method is new and can be applied to any plant that is subjected to any stress because generally, plants produce callose in response to stress.” Mr. Mustafa further states that the method is convenient because, if necessary, samples can be processed between a few hours to one year (or even longer), which allows exchange of materials between laboratories and countries when needed, something that was not possible before. The S-ELISA method used 96-well plates which allows high throughout studies, hence, hundreds of samples can be analyzed simultaneously within a few days.
This study has been published as a Protocol by Multidisciplinary Publishing Institute (MDPI) in an open access journal, “Methods and Protocols”, in the section: Biochemical and Chemical Analysis & Synthesis and is freely accessible on https://www.mdpi.com/2409-9279/5/4/54/htm.
The research was funded by the Bill and Melinda Gates Foundation through the National Agricultural Research Organization (NARO), Uganda. The project had partners including the International Institute of Tropical Agriculture, the Alliance for Bioversity International and International Centre for Tropical Agriculture (CIAT) and CABI.
For more details, contact;
- Prof. Arthur Tugume, Lead Scientist of the study, College of Natural Sciences, Makerere University, Email: arthur.tugume@mak.ac.ug, Tel: +256772514841
- Mr. Abubakar S. Mustafa, Co-Author and PhD student on the study, Email: mustafa.abubakar.sadik@gmail.com, Tel: +256702813233
- Hasifa Kabejja, Principal Communication Officer, College of Natural Sciences, Makerere University, Email: pr.cns@mak.ac.ug, Tel: +256774904211
Natural Sciences
Enhancing Data Quality: NutriFishPlus Research Assistants Retooled Ahead of Baseline Survey
The NutriFishPlus Project team successfully conducted a one-day capacity-building training for its research assistants, centered on the Monitoring, Evaluation, and Learning (MEL) framework and the household survey tool. Held on 13th January 2026 at Ridar Hotel Seeta in Mukono District, the training aimed to improve data collection, analysis, and learning processes to support evidence-based project implementation and impact assessment.
It was officially opened by Dr. Jackson Efitre, the Principal Investigator, who introduced the research assistants to the overall goal of the NutriFishPlus Project and the guiding principles of the International Development Research Centre (IDRC) Scaling Approach. In his remarks, Dr Efitre emphasized the importance of high-quality data in tracking progress, informing adaptive management, and scaling impact.
NutriFishPlus builds on the significant achievements of the ground-breaking NutriFish Project (2019–2023), which was funded by IDRC and the Australian Centre for International Agricultural Research (ACIAR) through the Cultivate Africa’s Future Fund Phase II (CultiAF2). Officially launched on 28th October 2025, the two-year and eight-month project seeks to scale proven innovations while deepening nutrition, livelihood, and gender outcomes within small pelagic fisheries.
The project aims to:
- Scale up the adoption of improved and sustainable fish processing technologies, including solar tent driers and raised racks, to reach new fishing communities across Uganda.
- Enhance market access and strengthen supply chain linkages for high-quality fish and fish-based products.
- Empower women and other vulnerable groups while strengthening community resilience through diversified income-generating activities.
Expected Outcomes:
NutriFishPlus is expected to deliver multiple socioeconomic and environmental benefits, including:
- Improved incomes and livelihoods for marginalized fishing communities, particularly women and youth.
- Enhanced health and nutrition outcomes through the development of diversified, market-embedded fish products.
- Establishment of sustainable and replicable fish processing and marketing models across Uganda and the wider East African region.
- Increased participation of women and youth in decision-making and benefit-sharing within the Small Pelagic Fishes (SPFs) value chain.
- Improved socioeconomic conditions and ecosystem health through participatory and scalable approaches.
Project Implementation and Partnerships
The project is implemented by the Department of Zoology, Entomology, and Fisheries Sciences at Makerere University, in partnership with Nutreal Ltd and Kati Farms Ltd under a public-private partnership. Project activities will be carried out at five landing sites across Lakes Victoria, Kyoga, and Albert.
The project team comprises:
- Dr. Jackson Efitre (Principal Investigator)
- Dr. Dorothy Nakimbugwe (Co-PI from Nutreal Ltd)
- Ms. Lovin Kobusingye (Co-PI from Kati Farms Ltd)
- Dr. Robinson Odong (Project Officer, Department of Zoology, Entomology and Fisheries Sciences, Makerere University)
- Ms. Juliet Ogubi Nafula (M&E Expert)
- Ms. Nelly Badaru (Gender Specialist)
- Mr. Eugene Swinnerstone Miheso (IT Expert)
- Mr. Maurice Ssebisubi (Kati Farms Ltd)
- Ms. Hasifa Kabejja (Communication Officer)
- Ms. Eve Akongo Ampa (Project Administrator)
Strengthening MEL and Data Quality
During the training workshop, Ms. Juliet Ogubi Nafula guided the team through the NutriFishPlus MEL framework, highlighting its objectives, indicators, results chain, and reporting requirements. Particular emphasis was placed on the role of high-quality data in facilitating project learning, adaptive management, and accountability to donors and beneficiaries.
A key highlight of the training was a hands-on orientation to the household survey tool, led by Mr. Eugene Swinnerstone Miheso. Participants were taken through the structure of the questionnaire, which covers household demographics, nutrition and dietary diversity, fish consumption, livelihoods, value addition, gender dynamics, and food security. Practical sessions enabled the research assistants to practice administering the tool, apply effective probing techniques, and address common field-level challenges.
The training underscored critical ethical considerations in household data collection, including informed consent, confidentiality, and respectful engagement with respondents. Data quality assurance measures such as accurate data recording, use of digital data collection platforms, and daily review protocols were discussed to ensure consistency and reliability.
In his closing remarks, Dr. Efitre emphasized that well-trained research assistants are central to generating credible evidence that reflects the lived realities of beneficiary households. “This training is a vital investment in strengthening our MEL system and ensuring that the evidence we generate informs learning, accountability, and improved project outcomes,” he noted.
The participants expressed gratitude for the interactive and practical nature of the training, noting that the sessions significantly enhanced their confidence and readiness for fieldwork. The training concluded with a question-and-answer session and a brief assessment to confirm participants’ understanding of the MEL framework and household survey tool.
Pre-testing of the household survey tool
Following the training, the project team conducted a pre-test of the household survey tool at Kasenyi Landing Site in Entebbe on 19th January 2026, ahead of the baseline survey. The baseline data collection will be carried out across five landing sites: Katosi (Mukono District), Kikondo (Buikwe District), Bangaladesh and Kayago (Amolatar District), and Dei (Pakwach District), spanning Lakes Victoria, Kyoga, and Albert.
Details on the project at: https://news.mak.ac.ug/2025/10/mak-conas-launches-nutrifishplus-project/
Dr Oruru Bosco has been appointed Acting Head of the Department of Physics at the College of Natural Sciences (CoNAS), Makerere University effective 1st January 2026. Dr Oruru takes on from Dr Okello Denis who has served in the same position since January 2022. He becomes the 10th Head of Department since its establishment. His predecessors include Prof. David Thomson, Prof. John Odeke Ilukor, Prof. Eldad J.K. Banda, Prof. Ezra K.S. Mugambe, Prof. Yusto Kaahwa, Prof. Ezra M. Twesigomwe, Prof. Florence Mutonyi D’ujanga, Prof. Winston Tumps Ireeta, and Dr. Denis Okello.
Dr. Oruru holds a PhD in Physics from the University of the Free State, South Africa, an MSc in Physics from the University of the Free State, a BSc in Astrophysics and Space Science from the University of Cape Town, a BSc in Education (Physics and Mathematics) from Mbarara University of Science and Technology, a Postgraduate Diploma in Public Administration and Management from Uganda Management Institute, and a Diploma in Secondary Education (Physics and Chemistry) from the Institute of Teacher Education, Kyambogo.
He has served the Department in various capacities as a Lecturer, Senior Lecturer, Graduate Supervisors Trainer, and Secretary of the Departmental Research and Teaching Programmes Committee. Dr. Oruru has supervised and examined several PhD, MSc, and BSc students at Makerere and other institutions, including Kyambogo University, Mbarara University of Science and Technology, the South African Institute of Physics, and the University of the Free State. His research interests include accretion onto magnetized compact objects (e.g., X-ray binaries and cataclysmic variables), exotic phenomena such as gamma-ray bursts, supernovae explosions, and active galactic nuclei, African cultural astronomy, and radiation physics.
At the handover ceremony held today, 6th January 2026, Dr. Denis Okello congratulated Dr. Oruru on his appointment and expressed gratitude to the College and University administration for the support accorded to him during his tenure. He also thanked the department staff for their cooperation and commitment. “The support I received enabled me to serve successfully and build upon the achievements of my predecessors. I leave the Department in a much improved state, with well-established infrastructure, including a lecture theatre, classrooms, teaching, research, and ICT laboratories, as well as a departmental library stocked with textbooks relevant to our programmes.”
During his tenure, Dr. Okello oversaw the review of the Bachelor of Science programmes (BSCI and BSCX) to align with the competence-based curriculum. The Department also acquired 55 computers through government funding, in addition to three iPads donated through the College. Renovations were carried out on the interior and exterior of the Department. Staffing improved, with 17 teaching staff currently in place out of the approved 27 positions. Recruitment efforts continue to fill the vacant positions.
Dr Okello appealed to staff to continue working together to further strengthen the department, and pledged support to the new head.
In his remarks, Dr Oruru thanked the staff and university management for entrusting him with the leadership of the Department. ““I am delighted to work alongside a team of dedicated professionals who understand their responsibilities and remain focused on our shared goals. I step into this position not merely as a leader, but as a representative of an exceptional team. With the guidance and support of my predecessors, I am confident that we will continue to uphold and build upon the department’s tradition of excellence.
The Dean, School of Physical Sciences, Dr Michael Owor commended both outgoing and incoming Heads for their commitment to the growth and success of the Department.
Natural Sciences
Jinja Fish Festival: Mak Recognized for its outstanding contribution to the Development of the Fisheries Sector
Makerere University, through the College of Natural Sciences (CoNAS), has been recognized for its outstanding contribution to the development of the fisheries sector in Uganda. The award was presented during the Sustainable Fisheries, Aquaculture, and Environmental Awards Ceremony at the 7th edition of the Jinja Fish Festival, held on 6th–7th December 2025 at Across the Nile Resort, along the banks of the River Nile in Njeru Municipality.
Renowned for showcasing diverse fish cuisines and cultural exhibitions, this year’s festival aimed to promote knowledge exchange, raise awareness, and highlight recent developments around Lake Victoria.
Launched in 2018, the Jinja Fish Festival has become a key platform for the public and stakeholders to learn about sustainable fisheries and the vital role of fish in livelihoods and nutrition. It also provides an avenue for fisheries stakeholders to deliberate on strategies to improve and sustain the sector.
The event includes the Lake Victoria Marathon, Nalubaale boat rowing competitions, a high-level fisheries forum, workshops, and exhibitions. It is organized by the Lake Victoria Fisheries Organization (LVFO) in partnership with the Directorate of Fisheries Resources, Sustainable Fisheries Initiative (SFI), Uganda Fish Processors and Exporters Association (UFPEA), and the International Fish and National Organizing Committee. It is funded by GIZ–SAF Project, the Food and Agriculture Organization (FAO), the International Fund for Agricultural Development (IFAD), and Enabel.
National Fisheries and Aquaculture Forum
A key highlight of the festival was the National Fisheries and Aquaculture Forum, themed “Promoting Sustainable Fisheries and Aquaculture through Ethical and Inclusive Blue Economy Practices.” The forum featured panel discussions on:
- Aligning Uganda’s Blue Economy with global and regional fisheries management frameworks.
- Adoption of climate-smart technologies in fisheries and aquaculture.
- Integration of business strategies and human rights principles into the fisheries and aquaculture value chain.
- Sustainable development of the fisheries and aquaculture sector.
Participants emphasized the importance of strengthening value chain governance, scaling up innovations, and aligning initiatives with youth employment opportunities.
Delivering the keynote address, Mr. Richard Ogutu-Ohwayo, Coordinator at African Lakes Network and Independent Researcher, highlighted the urgent need to address “dirty fish” corruption, improve fisheries resource management through effective policies, and increase human resource capacity within the sector. Dr. Odongokara Konstantine, a Research and Policy Expert, emphasized domesticating international policies into Uganda’s aquaculture practices and building stakeholders’ capacity to access regional and global opportunities.
NutriFishPlus Project Participation in the Festival
The NutriFishPlus Project team, along with students from CoNAS participated in the 7th edition of the Jinja Fish Festival on 6th and 7th December 2025. Led by Ms. Nelly Badaru, Gender Expert on the Project and Ms. Juliet Ogubi Nafula, PhD student in the Department of Zoology, Entomology, and Fisheries Sciences at CoNAS, the team showcased products developed under the project and educated participants on the nutritional benefits of small pelagic fishes.
In its first phase, titled NutriFish, Makerere University researchers, led by Dr. Jackson Efitre, in collaboration with the National Fisheries Resources Research Institute (NaFIRRI – NARO), NUTREAL Limited, and McGill University, Canada, developed five high-value nutritious products, including baby food, sauce, maize meal, snacks, and seasoning, using Mukene (silverfish). The team also published a Mukene recipe book, containing 16 recipes rich in protein, calcium, zinc, and iron.
Through the project, fishers gained access to loans for purchasing boats and fish containers that improve hygiene and product quality. Two enterprise fishing groups, with over 70 boats, were established to ensure consistent supply and standards. Researchers also developed the eCAS system, an application for tracking fish catches. By September 2022, 8,960 catch records had been transmitted through the system, which is now used by over 200 stakeholders, including the Directorate of Fisheries Resources and the Fisheries Protection Unit, helping prevent overfishing.
Other achievements registered under the project included the development of solar tent driers – greenhouse-like structures that reduce the risk of contamination and provide clean and efficient storage and drying, especially during the wet season subsequently reducing post-harvest losses. The project conducted awareness training for 326 people (200 women) to reduce gender inequalities. As a result, domestic violence dropped by 30%, and the number of women in the fish value chain increased. The project also trained eight Graduate students, who have gained valuable skills and knowledge thus boosting critical human resource needs in the fisheries sector.
Other exhibitors at the festival included (NaFIRRI – NARO), FAO, INNOECOFOOD Project at Makerere University, the International University of East Africa, Busitema University, SEETA University (Science, Environment, Engineering, Technology, and Agriculture), Yalelo Uganda, and Preventive Care International, all showcasing emerging technologies and innovations in fisheries.
NutriFishPlus Phase II
Funded by the International Development Research Centre (IDRC) and the Australian Centre for International Agricultural Research (ACIAR) under CultiAF2, the NutriFishPlus Project aims to scale up Phase I activities to further enhance incomes and livelihoods of Uganda’s fishing communities.
Launched on 28th October 2025, the 32-month project will run until March 2028. Its objectives include:
- Scaling up sustainable fishing technologies, including solar tent driers and raised racks, to reach new communities.
- Strengthening market access and supply chain linkages for high-quality fish and fish-based products.
- Empowering women and vulnerable groups through diversified income-generating activities.
Implemented by the Department of Zoology, Entomology, and Fisheries Sciences at Makerere University, in collaboration with Nutreal Ltd and Kati Farms Ltd, the project is led by Dr. Jackson Efitre (Principal Investigator).
Expected outcomes include:
- Improved livelihoods and incomes for marginalized fishing groups, especially women and youth.
- Better health and nutrition through diversified, market-driven fish products.
- Sustainable fish processing and marketing models replicable across Uganda and the East African region.
- Enhanced participation of women and youth in decision-making and benefit-sharing within the Small Pelagic Fishes (SPFs) value chain.
- Strengthened community resilience and ecosystem health through participatory approaches.
Project activities will be conducted at five landing sites around Lakes Victoria, Kyoga, and Albert: Katosi and Kikondo (Mukono and Buikwe districts), Bangaladesh and Kayago (Amolatar District), and Dei (Pakwach District).
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