Natural Sciences
Mak Scientists Unveil New Method of Measuring Stress Levels in Plants
Just like animals and human beings, plants too can be stressed. Whereas one may easily detect stress in animals and humans basing on their behavior, plant “behavior” is not easy to interpret.
Why should we be concerned about stress in plants?
Plants under stress cannot reproduce or grow well because they have to divert much of their physiological energy to fighting stress. If the plants are critical food crops, this will lead to perpetual food shortages because their energy resources are spent fighting stress.
New study on measuring stress in plants
A new study titled “Sandwich Enzyme-Linked Immunosorbent Assay for Quantification of Callose” and led by a team of scientists at the Department of Plant Sciences, Microbiology and Biotechnology at Makerere University in collaboration with the University of California, Davis, USA, shows how stress in plants can be measured. According to Prof. Arthur Tugume, the lead scientist of this study and expert in plant diseases, when plants are stressed, they produce numerous molecules within their cells mostly as means of adapting to stress or avoiding the impact of stress. “For example, plants produce reactive oxygen species like hydrogen peroxide, superoxide ions, and hydroxyl ions. These reactive oxygen species are produced rapidly and act as rapid messengers in the plant tissues to activate additional responses spreading over the entire plant body. This helps the plant to withstand or avoid the impact of stress”.
Dr. Ssenku E. Jamilu, a plant physiology expert on the team explains that in plants, stress can be induced by many factors such as pollution in the soil and atmosphere, high soil salinity (salt stress), excessive lack of water (drought), extreme temperatures, lack of oxygen (anoxia), excess radiation, mechanical injury by wounding or by pests that feed on the plants, and infestation by disease-causing microorganisms (pathogens). “Such factors are worsened by climate change, implying the importance of measuring their impact on individual plants to guide plant breeding programmes to ensure sustainable food production in the face of climate change”.
The research indicates that reactive oxygen species set in motion additional processes to ensure limited impact of stress on the plant. For example, a unique carbohydrate, named “callose” starts to accumulate in large quantities within plant cells as a means of fortifying plant cells. Callose differs from the other usual plant carbohydrates such as starch or cellulose because of the way its structures are formed. Also, the production of callose gets increased during stress. Callose acts as a road-block to any pathogen such as bacteria by limiting bacterial movement that would otherwise ease attack on other tissues or cells.
“If we can artificially induce the stress on the plant, and then measure the amount of reactive oxygen species or callose or any other responses in the plant, we should be able to directly and quantitatively measure the stress,” Prof. Tugume explains.
He however notes that callose participates in many other normal developmental processes of plants and for that reason, there is always some “housekeeping” callose in the plant tissues even without stress. “This means that one must be able to accurately and quantitatively distinguish between ‘stress-induced’ and normal ‘housekeeping’ callose”.
In this study, the researchers used young (2.5-months old) banana plantlets that had been generated from tissue culture at Kawanda Agricultural Research Institute. They then infected the plantlets with a bacterium called Xanthomonas campestris pv. musacearum. This bacterium induces so much stress on the banana plants resulting into a destructive disease known as banana xanthomonas wilt (BXW), the most destructive disease of bananas in East and Central Africa.
The banana leaves, pseudostems and corms were analyzed for callose and compared with the control plants that had been inoculated with water instead of bacteria. The analysis was done using a method called S-ELISA (Sandwich Enzyme-linked immunosorbent Assay), that was designed by scientists in this study. This method is specific to callose.
Callose-specific primary antibodies were used to coat the wells of 96-well microplates followed by incubation with callose extracts from tissues of stressed plants. Additional incubation with same primary callose-specific antibodies was done, and finally, incubation with a secondary antibody that is conjugated to an enzyme. When a substrate is supplied to the enzyme, colour changes were monitored. The presence of callose (and thus stress on the plants) was assessed quantitatively by measuring the intensity of color change resulting from enzymatic action on the substrate. This method proved effective in comparison to the gold standard method of detecting and quantifying aniline blue stained callose using fluorescence microscopy.
This research was part of the PhD studies for Mr. Abubakar S. Mustafa at Makerere University and University of California, Davis. According to Mr. Mustafa, his method is new and can be applied to any plant that is subjected to any stress because generally, plants produce callose in response to stress.” Mr. Mustafa further states that the method is convenient because, if necessary, samples can be processed between a few hours to one year (or even longer), which allows exchange of materials between laboratories and countries when needed, something that was not possible before. The S-ELISA method used 96-well plates which allows high throughout studies, hence, hundreds of samples can be analyzed simultaneously within a few days.
This study has been published as a Protocol by Multidisciplinary Publishing Institute (MDPI) in an open access journal, “Methods and Protocols”, in the section: Biochemical and Chemical Analysis & Synthesis and is freely accessible on https://www.mdpi.com/2409-9279/5/4/54/htm.
The research was funded by the Bill and Melinda Gates Foundation through the National Agricultural Research Organization (NARO), Uganda. The project had partners including the International Institute of Tropical Agriculture, the Alliance for Bioversity International and International Centre for Tropical Agriculture (CIAT) and CABI.
For more details, contact;
- Prof. Arthur Tugume, Lead Scientist of the study, College of Natural Sciences, Makerere University, Email: arthur.tugume@mak.ac.ug, Tel: +256772514841
- Mr. Abubakar S. Mustafa, Co-Author and PhD student on the study, Email: mustafa.abubakar.sadik@gmail.com, Tel: +256702813233
- Hasifa Kabejja, Principal Communication Officer, College of Natural Sciences, Makerere University, Email: pr.cns@mak.ac.ug, Tel: +256774904211
Natural Sciences
Mak-CoNAS Wins CAD 0.8 Million Grant to Scale-up Fish Processing Technologies & Empower Women in Uganda
The College of Natural Sciences (CoNAS) at Makerere University has been awarded a highly competitive research grant worth CAD 0.8 million to implement the “NutriFishPLUS” project, which aims to scale up innovative fish processing technologies, improve market access, and empower women in Uganda’s fishing communities to boost incomes and livelihoods.
This project builds on the significant achievements of the previous ground-breaking NutriFish project (2019–2023), funded by the International Development Research Centre (IDRC) and the Australian Centre for International Agricultural Research (ACIAR) through the Cultivate Africa’s Future Fund Phase (CultiAF2). The achievements included: 1) establishing group savings schemes to improve access to capital by women and youth; 2) piloting solar tent dryers for processing silverfish (mukene), resulting in doubling of women’s incomes and tripling of the shelf-life to almost five months; 3) developing and test-marketing certified, nutrient enriched fish products, including baby food, sauce and fortified maize meal; 4) conducting comprehensive social and behavioural change interventions, leading to increased women’s participation in profitable ventures and 30% reduction in domestic violence in intervention areas. Despite these achievements, gaps still remain with regard to increasing production of high-quality Small Pelagic Fishes (SPFs) through adoption of solar tent dryers and raised drying racks; enhancing capacity of men, women and the youth in processing, packaging, branding and marketing; improving access to capital and lucrative markets for fish and fish products; and empowering women, youth and other marginalized groups in the small fish value chain; and strengthening resilience of fishing communities through diversified income streams.
NutriFishPLUS will be implemented by the Department of Zoology, Entomology and Fisheries Sciences, CoNAS, in collaboration with two private companies (Kati Farms and Nutreal) through a public-private partnership. The Principal Investigator is Dr. Jackson Efitre and his team will focus on scaling-up the use of improved, sustainable fish processing technologies such as the solar Tent dryers and raised racks to new communities across Uganda; enhancing market access and supply chain linkages for high-quality fish and fish products; as well as deepening women’s empowerment and strengthening the resilience of fishing communities through diversified income streams. The project is expected to run for September 2025- March 2028. The expected outcomes include: enhanced incomes and livelihoods for marginalized fishing groups, particularly women and youth; improved health and nutrition for vulnerable groups through diversification of fish products that are embedded in the market with strong supply chain linkages; sustainable fish processing and marketing models that can be scaled across Uganda and the East African region; improved women and youth participation in decision making and control of benefits in the SPF value chains; and improved socioeconomic conditions and ecosystem health through participatory, scalable approaches.
“Winning this competitive grant is an incredible opportunity for the team to solidify the achievements of the first phase as the funding enables us to move beyond research to embed these nutritional and technological solutions into the livelihoods of local communities,” said Dr. Efitre. “I am privileged to lead this impactful work on behalf of Makerere University. Scaling up these tested, climate-responsive technologies as well as empowering the women and youth will secure better nutrition and more sustainable livelihoods across fishing communities in Uganda.”
The project is set to be launched tomorrow, Tuesday, 28th October 2025 by the Acting Deputy Vice Chancellor, Finance and Administration and Principal, CoNAS, Prof. Winston Tumps Ireeta.
Please see below for details on the project.
Details on the previous project: https://news.mak.ac.ug/2023/03/nutrifish-project-registers-significant-achievements/
Agriculture & Environment
Ugandan Local Government Officials Trained on Environmental Valuation, Accounting, and Climate Resilience
Entebbe, Uganda – September 19, 2025
More than 20 local government officials from Kampala Capital City Authority (KCCA), Entebbe Municipality, and the districts of Wakiso, Mukono, and Mpigi have concluded a two-day intensive training on Environmental Valuation, Accounting, Evaluation, and Climate Change Mitigation and Adaptation.
Held at Central Inn Hotel in Entebbe from September 18–19, the workshop was organized by the Environment for Development (EfD) Makerere University Centre and funded by the Makerere University Research and Innovation Fund (Mak-RIF). The objective was to build local capacity in environmental governance and integrate natural capital and climate risks into local development planning.
The training was facilitated by Dr. Aisha Nanyiti, Dr. John Sseruyange, Dr. Peter Babyenda and Dr. Nick Kilimani- all from Makerere University.
Natural Resources Must Be Valued and Protected
Opening the workshop, Prof. Edward Bbaale, Director of the EfD-Mak Centre and Principal Investigator of the project, described the training as timely, given Uganda’s growing environmental and climate challenges.
“Uganda is already experiencing the adverse effects of climate variability—on agriculture, health, water resources, and the economy,” Prof. Bbaale noted. “This training empowers our environmental officers to use economic evidence to defend natural resources against competing land uses.”
He emphasized the need to monetize ecosystem services like wetlands, forests, and biodiversity to inform land-use decisions.
“A forest cleared for sugarcane may appear to create jobs, but when you account for its carbon sequestration, rainfall formation, and tourism potential, its long-term value far outweighs the short-term economic gains.”
Prof. Bbaale also urged that natural capital be included in Uganda’s national accounting systems to achieve sustainable and equitable development.
He thanked local government leadership for releasing their staff for the training and praised the collaboration between academia and government.
“You are not just trainees — you are future champions of environmental stewardship in your districts,” he told the participants.
Environmental Degradation Threatens Public Health
Representing the Chairperson of Mak-RIF, Dr. Sabrina Kitaka underscored the public health consequences of environmental degradation, linking it to disease outbreaks and natural disasters.
“We have just seen a 14-year-old girl in Lwengo contract Crimean-Congo hemorrhagic fever due to increased human-wildlife contact — a direct result of environmental encroachment,” Dr. Kitaka warned.
She connected rising flash floods, deforestation, and urban sprawl to poor environmental management and called for cross-sectoral awareness of environmental risks.
“This training is not just for environmentalists. Planners, economists, and health professionals must also be equipped with this knowledge,” she said.
Dr. Kitaka applauded the EfD-Mak team for using local experts, not foreign consultants, in delivering the training.
“We must train Ugandans to solve Uganda’s problems. That’s the spirit of Mak-RIF.”
She also revealed that the Government of Uganda has funded over 1,300 research projects through Mak-RIF over the last five years and called for continued investment in locally driven innovation.
“Like Oliver Twist, we ask for more. Science must be funded to help Ugandans solve Ugandan problems.”
Experts Present Practical Tools for Sustainable Planning
Localized Climate Action Is Urgent
Dr. Aisha Nanyiti urged participants to design district-specific climate policies, differentiating between mitigation (addressing the causes) and adaptation (responding to the impacts).
“Climate action begins at the local level. Districts must create context-appropriate bylaws and planning strategies to reduce vulnerability to floods, droughts, and other climate shocks.”
She emphasized that Uganda’s local governments are key to meeting national targets under NDP IV and Vision 2040, and that climate change must be addressed in all sectors — from health to urban planning.
Environmental Evaluation is a Safeguard
Dr. Sseruyange warned that development projects are at risk of failure if environmental assessments are not properly conducted.
“Roads, schools, and hospitals may be destroyed by floods or landslides if we ignore the environment. Planning must consider both the impact on nature and the reverse impact from degraded ecosystems.”
He called on local environmental officers to assert their roles in safeguarding both the environment and infrastructure investment.
“Neglecting environmental oversight may undo everything we build.”
What is Not Valued is Easily Destroyed
Dr. Peter Babyenda introduced participants to valuation tools like Contingent Valuation Method (CVM) and the Travel Cost Method, which help quantify the economic value of forests, wetlands, and biodiversity.
“These tools inform cost–benefit analysis, budgeting, and resource allocation. If we don’t value our natural assets, we risk losing them permanently.”
He stressed that environmental protection is not only an ecological issue, but also an economic, social, and political matter. He encouraged integration of valuation results into budgeting and planning under frameworks like the National Environment Act (2019).
Local Officials Call for Continued Engagement
Ibrahim Muwanguzi, Environmental Officer from Wakiso District, praised the training as a vital step toward mainstreaming environmental valuation into local and national development processes.
“Most government officials focus solely on economic returns, ignoring the critical but intangible value of natural resources,” he said.
He cited a recent case during Mpigi Expressway construction where a culturally significant tree sparked debate over its monetary value — from UGX 100,000 to UGX 100 million — due to lack of valuation standards.
“If services like flood control were quantified in shillings, it would help justify preservation of wetlands and forests,” he noted.
Muwanguzi also emphasized the need for environmental economists in government roles, urging stronger collaboration between academia and policy.
“We have graduates in natural resource economics. Let’s integrate them into our public service.”
Let This Not Be the Last
Tonny Mwidyeki, District Natural Resources Officer for Mpigi, delivered the vote of thanks on behalf of all trainees, emphasizing the long gap since the last such training.
“For many of us, it’s been years since we had a refresher like this. If we continue to have such sessions, we will serve our people and protect our environment better.”
He thanked the facilitators from Makerere University for the two-day sensitization and urged that such capacity-building workshops be held more regularly.
“From here, we go back not just informed, but ready to lead change.”
Time to Act is Now
Closing the training, Dr. Sseruyange, speaking on behalf of the EfD-Mak Director, reminded participants that environmental degradation has been decades in the making, and action must no longer be delayed.
“Development depends on the natural world. Let us act now — from our households to our districts — to reverse the damage and secure our future.”
He thanked the participants for their active engagement and promised continued collaboration between Makerere University, local governments, and national stakeholders to promote evidence-based environmental policy.
About EfD-Mak and Mak-RIF
The Environment for Development (EfD) Centre at Makerere University is part of a global network of research institutions focused on environmental economics, coordinated by the University of Gothenburg, Sweden.
The Makerere University Research and Innovation Fund (Mak-RIF) is a Government of Uganda-funded program supporting local research to address national development challenges. Since inception, Mak-RIF has supported over 1,300 research projects across disciplines.
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