The Project is supported by the Government of Uganda through the Makerere University Research and Innovations Fund (Mak-RIF).

Overview

African swine fever (ASF) is a highly contagious viral haemorrhagic disease of domestic pigs; and has recently spread to new areas including Eastern Europe and in Asia as far as Papua New Guinea. This disease has neither vaccine nor treatment and is currently a big challenge to many smallholder pig farmers in Uganda. In Uganda, ASFV genotype IX has been reported to cause outbreaks on a regular basis and is an impediment to development of the swine industry. The disease was previously reported with a mortality of 100%. However different forms of the disease have recently been observed in domestic pigs. Indeed, some studies have shown that pigs that survive ASF become resistant to the same viral isolates although they do not produce classical neutralizing antibody. These observations could have the key for vaccine development strategies.

The research project

Through a project titled “African swine fever virus (ASFV) severity and viral genomic structural features: opportunities for development of a vaccine in Uganda”, a team of researchers led by Prof. Charles Masembe from the Department of Zoology, Entomology, and Fisheries Sciences at Makerere University registered the need to correlate clinical studies of ASF virus with full genome sequences and virus structural data to identify potential candidate genes for vaccine development. The team set out to isolate and characterise highly virulent and low virulent ASF viruses circulating in the swine population in Uganda using next-generation and Electron Microscopy to inform the development of ASFV vaccines and other therapeutic strategies. The team also set out to sequence the whole genome of low and high-virulence African swine fever viruses circulating in the domestic pig population in Uganda; compare the whole genome sequences of low and high-virulence ASF viruses in Uganda, and to find genomic variations and their effects on the virulence and viral evolution. Whole blood and organ tissue samples together with clinical-epidemiological information, were collected from acute and chronic cases of pigs in reported ASF outbreaks in the districts of greater Masaka, Greater Gulu, Mukono, Wakiso, Kakumiro, and additionally in other districts were outbreaks were reported during the study period. The project was supported by the Government of Uganda through the Makerere Research and Innovations Fund (MakRIF). Other members on the project are; Assoc. Prof. Vincent Muwanika (Co-PI), Dr. Rose Ademun (Scientist), Dr. Mathias Afayoa (Scientist), Dr. Tony Aliro (Field Scientist), Mr. Peter Ogweng (Field Scientist),  Mr. Mayega Johnson Francis (Lab Technologist), Okwasiimire Rodney (Lab Technologist), and Mr. Bright Twesigye (Administrator).

Dissemination of the research findings

On 15^th May 2024, the project team held a workshop at Doves Nest Hotel in Gulu District to disseminate the research findings, and to fact check by mapping pig farms, abattoirs, slaughter slabs, and pork restaurants in both Gulu District and Gulu City for on-going and future research.

The workshop was officially opened by the Assistant Chief Administrative Officer (CAO) of Gulu District, Mr. Okidi Cosmas James on behalf of the CAO. In his remarks, Mr. Okidi appreciated the project team, noting that the workshop was timely since piggery as a poverty alleviation programme had been embraced by most farmers as a replacement for cattle. Sharing his experience, he expressed concern over the increasing threat of ASF, a factor he said was undermining the growth of the swine industry in Uganda.

During the meeting, the Project PI, Prof. Charles Masembe presented the status of ASF research in Uganda and globally, highlighting the complexity of ASF, dangers of ASF, ASF diagnostic advances, importance of keeping pigs and scientific efforts to control ASF, including the advances in the development of the ASFV vaccine. 

Other presentations focused on ASF control and prevention mechanisms, potential role of production systems in ASF transmission, community involvement in disease control, spatio-temporal spread of ASF at the wildlife-livestock interface, the role of carrier pigs in ASFV transmission and maintenance, biosecurity implementation and challenges. All these presentations evoked discussion points from the participants. Participants expressed the need to understand how ASF is transmitted, and the extent to which farmers can trust veterinarians since they are considered to spread the disease. They highlighted the need to institute quarantine during ASF outbreaks and called for sensitization on the acceptable methods for disposing off infected pork. Another concern arising from the meeting was the need to understand if ASF is zoonotic and if not, why ASFV infected pork should not be eaten. Participants also sought guidance on why farmers should continue to keep survivor pigs if they spread ASFV, and why the government has not constructed slaughter slabs in all villages to control the spread of ASFV.

Recommendations by participants;

1. Strengthening community awareness through different production departments in the district and city by organizing workshops as closer to the farmers as possible for example at every parish or ward.
2. Makerere University ASF research team should collaborate with Gulu University, Faculty of Agriculture and Environmental Studies so that ASFV diagnosis and research is jointly done at the University which is nearer to the farmers for quick response to ASF outbreaks.
3. City, District, and sub-county leaders should formulate a by-law to enforce the implementation of ASF control and prevention methods.

During plenary, participants were tasked with mapping pig farms/farmers, abattoir/slaughter slabs and pork restaurants in their respective sub-counties in Gulu District and Gulu City, in addition to generating a comprehensive farm/farmer list in the two areas. Mapping was successfully done and presented for purposes of clarity by each team representing a particular sub-county. The participants were then given a template to populate on the farm/farmer list since they could not recall all farms/farmers name and their mobile phone contacts.

Remarks by Gulu District RDC

In his closing remarks, the RDC of Gulu District, Mr. Oryem Auric thanked the research team for choosing the district for the workshop, noting that the feedback from the participants indicated that the workshop was very beneficial to them. He pledged to dedicate some of the free airtime offered to his office on Radio Mega, a popular FM Radio Station in Gulu City affiliated to Radio Uganda, towards the popularization of the project activities. He reiterated that the objectives of the project were in line with the current Government of Uganda programme on wealth creation through the Parish Development Model and the 4 acre model of which piggery is one of the means recommended for poverty alleviation by the President of Uganda. He called on the farmers to embrace and utilize the knowledge acquired to improve their farms and increase their household incomes.

View on CoNAS

The College of Natural Sciences (CoNAS) is one of the 10 constituent Colleges of Makerere University with over 2,000 students and 153 members of staff spread across 2 schools and seven departments namely: School of Physical Sciences (Physics, Chemistry, Mathematics, Geology and Petroleum Studies) and School Biosciences (Departments of Plant Sciences, Microbiology and Biotechnology; Department of Zoology, Entomology and Fisheries Sciences; and Department of Biochemistry and Sports Sciences). The College is the cradle of basic sciences at Makerere providing a foundation for all applied sciences offered at the University. This report is a presentation of the performance of the College in 2023.

Key highlights include performance of the College at the 74^th graduation of Makerere University, the award of an Honorary Doctor of Science to Prof. Leif Abrahamsson for his contribution to the development of Mathematics in the East African region, the development of an E-Supervision Web Portal (https:/mastersresearch.ug/) to support the tracking of Masters Research supervision processes, the formation and operationalization of the Biology Society of Uganda (BioSU), support rendered towards the development of aquaculture in the country, the development of five high-value nutritious products (baby food, sauce, maize meal, snacks, and seasoning) using Mukene through the NutriFish Project, the development of an application for tracking fish catches as a measure to prevent overfishing, development of solar tent driers – greenhouse-like structures that reduce the risk of contamination – provide clean and efficient storage and drying, especially during the wet season subsequently reducing post-harvest losses, equipping students and members of the general public with skills needed in the installation and maintenance of solar energy Photovoltaic systems and biogas plants, the Joint African- Nordic Conference in Mathematics, as well as the 2023 Eastern Africa Algebra Research Group and Women in Sage workshops. The report also highlights the research grants received in the course of the year, conferences held, community engagements, activities by students, awards received by the different members of staff, staff publications, and matters of Human Resources at the College.

Management conveys appreciation to all development partners and the Government of Uganda for the support extended towards the different projects at the College. We also thank the University Council and Management for creating a conducive environment for both staff and students to explore their full potential.

_{By Dr. Alice Nabatanzi}

As we train Scientists we ought to equip them with skills that will help them survive beyond the Laboratories, beyond University, and beyond Academia. Thus, the need to Bioprospect and acquire product development skills.

I am therefore proud of what my 2024 Biotechnology Product Development Class has been able to achieve.

Following bioprospecting and entrepreneurial training, students developed the following products: Exotic beard oil, Herbal cough mixture, Power candy, Laundry bar soap, Moringa snack bars, Prudy Organic hair dye, Hair food, Syphilis remedy, Cornstarch based bioplastic, Nash probiotic drink, Dental care herbal mouthwash, Rosemary – lemongrass infused vinegar, Tea tree hand wash, Scented candles, Aloe fruit jam, Herbal bloom shower gel, Custard, Beetroot yoghurt, Dewormer, Stomach pain killers, Liquid organic fertilizers, Face scrub, Mosquito repellant scented air diffuser, Underarm whitening oil, Essential oil deodorant, Ria hair conditioner, Shower gels, Crochet jacket among others.

I am confident that with subsequent incubation, start-ups will emerge.

“The future belongs to those who learn more skills and combine them in creative ways.”

Robert Greene

“Build your skills not your resume.”

Sheryl Sandberg

Dr. Alice Nabatanzi is a Lecturer of Phytomedicine and Nutraceuticals in the Department of Plant Sciences, Microbiology and Biotechnology, School of Bio-sciences, College of Natural Sciences (CoNAS), Makerere University.

Photo Gallery of some of the products