Natural Sciences
Banana’s Large Plant Body Size an Advantage to Disease Control – Mak Scientists
Unlike for plants, in animals especially humans, body mass index (BMI, which is a person’s weight divided by the square of height) is a measure of physical health and pre-disposure to conditions like obesity. BMI does not make sense in plant health because of differences between plant and animal physiological systems.
However, large body size in plants may have some advantages. Apart from controlling a larger proportion of available resources and space within crowded vegetation, what other advantage does a large plant body size offer to an individual plant?
The banana’s plant body architecture
From the botanical point-of-view, the banana plant is a gigantic herb. A plant that is a herb or “herbaceous” is unable to undergo “secondary growth” and cannot form wood during its vegetative development.
The banana plant springs from an underground “true stem”, also called the “corm” or “rhizome”, to form a false stem, also called a “pseudostem” of 2-7 m height. The pseudostem is composed of the basal portions of leaf sheaths and is crowned with a rosette of 10 to 20 oblong to elliptic leaves that sometimes attain a length of 2-4 m and a breadth of 70 cm.
In mature banana plants, true stem emerges at the top of the pseudostem and bends downward to become a bunch of 10 to 300 individual fruits, or fingers, grouped in clusters, or hands, of 3 to 22. The edible part of the bunch is the female. In contrast, the inedible distal part, including the purple-colored cone-shaped end (locally known in some Ugandan dialects as “omukanaana” or “empumumpu”) constitutes the male part of the bunch.
How is the giant banana size an advantage in disease control?
A new study, titled “Xanthomonas campestris pv. musacearum Bacterial Infection Induces Organ-Specific Callose and Hydrogen Peroxide Production in Banana” and led by a team of scientists at the Department of Plant Sciences, Microbiology and Biotechnology at Makerere University in collaboration with the University of California, Davis, USA, shows how the giant banana body size can be used to control banana Xanthomonas wilt (BXW) disease.
According to Prof. Arthur Tugume, the lead scientist of this study and expert in plant pathology, when plants get infected, they respond instantly by implementing different strategies that limit the multiplication and/or mobility of the disease agents (pathogens). “For example, plants rapidly produce reactive oxygen species (ROS) such as hydrogen peroxide, superoxide ions, and hydroxyl ions. These ROS act as rapid messengers in the plant tissues to activate additional responses spreading over the entire plant body. This helps the plant’s distant tissues or organs to be aware and prepare advance defenses against the intruding pathogens”.
Prof. Dinesh-Kumar the project’s research collaborator based at the University of California-Davis, USA and expert in plant biology explains that “disease is a form of stress in plants and plants cannot perform well their biological functions when they are sick since they have to spend a lot of energy fighting against the disease. This is why disease control is important to enable plants grow well and yield high.”
The research indicates that ROS set in motion additional processes to ensure limited impact of disease and pathogens on the plant. For example, Hydrogen peroxide (H2O2), which is a ROS, has direct bactericidal, fungicidal or other anti-effects on the pathogens. Also, following H2O2 production, a unique plant carbohydrate, named “callose” starts to accumulate in large quantities within plant cells as a means of fortifying plant tissues. Callose differs from the other usual plant carbohydrates such as starch or cellulose because of the way its structures are formed.
Increased production of callose acts as a roadblock to any pathogen e.g., bacteria by limiting bacterial movement that would otherwise allow ease of attack on other tissues or cells at distant locations in the plant. “Although these plant defense responses are rapid, plant organs that are distant from the site of pathogen attack can be instrumental and block progression of bacteria or other pathogens by depositing callose in advance at strategic points” Prof. Tugume explains.
However, Prof. Tugume notes that callose participates also in many other normal developmental processes of plants, and for that reason, there is always some “housekeeping” callose in the plant tissues even without pathogenic infection. “This means that one must be able to accurately and quantitatively distinguish between ‘stress-induced’ and normal ‘housekeeping’ callose”, he adds.
How was the study done?
In this study, the researchers used young (2.5-months old) banana plantlets that had been generated from tissue culture at Kawanda Agricultural Research Institute. They then infected the plantlets with a bacterium called Xanthomonas campestris pv. musacearum (Xcm). This bacterium is the causative agent of banana Xanthomonas Wilt (BXW), the most destructive disease of bananas in East and Central Africa (ECA).
The banana leaves, pseudostems, corms and roots were analyzed for callose and compared with the control plants that had been inoculated with water instead of bacteria. H2O2 production was monitored by “DAB staining”, and by “spectrophotometry” while the analysis of callose was done by two methods: staining and visualization of callose using florescence microscopy, and using “Sandwich Enzyme-Linked Immunosorbent Assay” methods.
What did the researchers discover and how can it be used in BXW disease control?
This study revealed that the underground corm tissues assemble the stiffest resistance against BXW by depositing the highest concentrations of callose, while the pseudostem produced the highest quantities of H2O2. This is interesting for three (3) main reasons:
- Firstly, Xcm bacteria often enter through the leaves in regular plantation husbandry; hence, the corm being distant from leaves gives it an anatomical advantage in promoting the ability of lateral plants to escape Xcm infection.
- Secondly, the corm is an organ of perennation supporting vegetative and perennial continuity of the crop across seasons; hence it is charged in ensuring a disease-free next generation by severely constraining “mother-child transmission” of Xcm bacteria.
- Thirdly, the control of BXW now becomes easy when farmers are observant to the first aerial disease symptoms because Xcm is strongly constrained by bottlenecks in the pseudostem and corm.
Therefore, at the onset of aerial symptoms, diseased peudostems should immediately be removed by aseptically cutting them off at the corm without interfering with symptomless lateral shoots, which allows continuous food production and disease control to go on simultaneously. This is facilitated by the large size of the banana plant because at the onset of leaf symptoms (2.5 to 5 meters away from the corm), the bacteria have not yet arrived at the base of the pseudostem where the diseased plant can be cut off from the corm. This gives chance to a farmer to eliminate the infected pseudostems early (in 1 to 7 days) since the appearance of leaf symptoms.
This research was part of the PhD studies for Mr. Abubakar S. Mustafa at Makerere University and University of California, Davis. According to Mr. Mustafa, these discoveries make the management of BXW in banana plantations easy as long as the farmers are observant and act fast by removing diseased plants aseptically.
This study has been published by the American Phytopathological Society (APS) in an open access journal, “PhytoFrontiers”, and is freely accessible on https://apsjournals.apsnet.org/doi/full/10.1094/PHYTOFR-11-21-0073-R.
This study was funded by the Bill and Melinda Gates Foundation through the National Agricultural Research Organization (NARO), Uganda. The project had partners including the International Institute of Tropical Agriculture (IITA), the Alliance for Bioversity International and International Centre for Tropical Agriculture (CIAT) and Centre for Agriculture and Bioscience International (CABI).
For more details, contact;
Prof. Arthur Tugume
Lead Scientist
College of Natural Sciences (CoNAS)
Makerere University
Email: arthur.tugume@mak.ac.ug
Tel: +256772514841
Mr. Abubakar S. Mustafa
Co-Author and PhD student on the study
Email: mustafa.abubakar.sadik@gmail.com
Tel: +256702813233
Hasifa Kabejja
Principal Communication Officer
College of Natural Sciences (CoNAS)
Makerere University
Email: pr.cns@mak.ac.ug
Tel: +256774904211
Natural Sciences
BOKU University Charts New Collaboration Strategies with Mak’s Department of Zoology, Entomology & Fisheries Sciences
The Department of Zoology, Entomology and Fisheries Sciences at Makerere University on 11th February 2026 hosted a high-level delegation from the University of Natural Resources and Life Sciences, Vienna (BOKU), to discuss strategies for future collaboration in a partnership that has spanned five decades.
The meeting followed the BOKU team’s participation in the opening ceremony of the Promotion of Hybrid Renewable Energy Systems towards Electricity Access in Uganda project, an initiative designed to expand access to decentralized renewable energy solutions. The project, implemented under CEDAT, is funded by the Austrian Partnership Programme in Higher Education and Research for Development (APPEAR), a programme of the Austrian Development Cooperation administered by OeAD.
Discussions at the Department of Zoology, Entomology and Fisheries Sciences focused on deepening and broadening a historic collaboration that has shaped aquatic sciences, fisheries research, and environmental management in East Africa for half a century.
A Partnership Rooted in Limnology
The collaboration between Makerere University and BOKU traces its origins to 1975 with the establishment of the International Post-Graduate Course in Limnology (IPGL) by the late Heinz Löffler (1927-2006). The eight-month UNESCO-supported course, hosted in Lunz, Mondsee and Vienna, was designed to train scientists from developing countries in limnology – the study of inland waters.
In 1976, Dr. Gwahaba James Joshua of Makerere’s Department of Zoology, Entomology and Fisheries Sciences became the first beneficiary from the university. Two years later, Boniface Makanga attended the UNESCO course and went on to initiate a Marine Practical Course to the Indian Ocean in 1993. That programme has since grown into over 30 years of annual field activity in Mombasa, benefiting undergraduate and master’s students and strengthening hands-on marine science training at Makerere.
Over time, IPGL evolved into a vibrant International Joint Degree Master’s Programme in Limnology and Wetland Management. The programme has built capacity for 123 aquatic professionals – 56 female and 67 male, including 20 from Makerere University. Notably, 11 dedicated limnologists are currently based within the Department of Zoology, Entomology and Fisheries Sciences, a testament to sustained capacity development.
Expanding into Fisheries, Aquaculture and Regional Networks
The Mak-BOKU collaboration progressively expanded beyond limnology into fisheries, aquaculture, and broader freshwater ecosystem management.
Among the key initiatives was STRECAFISH (2015-2018), which focused on strengthening regional capacity in fisheries and aquaculture research and training to improve food security and livelihoods in Eastern Africa. The programme adopted a modular approach, trained 28 MSc students, supported five full PhDs and 11 MSc research projects, and facilitated 14 staff exchanges.
This momentum continued under COTRA (Collaborative Training in Fisheries and Aquaculture in East, Central and Southern Africa) from 2017 to 2023. COTRA enhanced staff and student mobility, supporting 24 master’s students (six credit-seeking and 18 degree-seeking), 12 doctoral candidates (four credit-seeking and eight degree-seeking), and 10 faculty and administrative staff. The initiative emphasized fit-for-purpose PhD training in aquaculture and fisheries to address food security challenges in Sub-Saharan Africa under the ACP-EU Cooperation Programme in Higher Education (EDULINK II), in partnership with institutions such as Lilongwe University of Agriculture and Natural Resources.
Ongoing collaborations include the Erasmus+ KA1 Learning Mobility of Individuals programme (2021-2027), which connects BOKU and Makerere with Egerton University, Kyambogo University, Addis Ababa University, Bahir Dar University and others. Additional joint initiatives include:
FreshNet: A higher education and research network for sustainable management of freshwater ecosystems in Eastern Africa, involving BOKU, Egerton University, Addis Ababa University, Bahir Dar University, EIAR and IHE Delft.
SWAQ-Uganda (2021-2026): Sustainable water quality management supporting Uganda’s development ambitions, implemented with IIASA, BOKU and Uganda’s Ministry of Water and Environment.
BIOGOLD (2024–2027): A project on biosorption for sustainable small-scale gold mining in Uganda, involving Kyambogo University, Makerere University, Gulu University, the University for Continuing Education and the University of Pretoria.
Collectively, these initiatives underscore a 50-year-old partnership that has built critical human resources at MSc and PhD levels in aquatic ecosystem management, conservation and utilization. The collaboration has also facilitated acquisition of essential research equipment, strengthened administrative capacity in project management, and nurtured a culture of knowledge sharing and innovation.
Meeting with the BOKU delegation
The BOKU delegation was led by the Rector, Prof. Eva Schulev-Steindl, a specialist in environmental law, and Vice Rector Assoc. Prof. Doris Damyanovic, an expert in landscape planning. They were accompanied by:
- Mag. Gerold Winkler, Coordinator of the Applied Limnology / Limnology and Wetland Master’s programme;
- Dr. Mathew Herrnegger, BOKU’s Africa Cooperation Country Coordinator and hydrology specialist; and
- Dr. Andreas Melcher, Africa-Uninet Coordinator from BOKU’s Institute for Development Research.
During the discussions with departmental staff, Dr. Akoll presented a detailed account of the partnership’s evolution and achievements, emphasizing its transformative impact on regional training and research capacity.
Charting the Next Chapter
As the partnership enters its sixth decade, both institutions are keen to expand into new frontiers. Key areas identified for future collaboration include:
- Enhanced staff and student exchanges;
- Joint research projects and co-publications;
- Introduction of short courses at the Makerere University Biological Field Station, Kibale beyond wildlife studies;
- Research on climate change and its effects on marine and freshwater resources;
- Advancement of basic biological research; and
- Strengthening fisheries research and innovation.
In his remarks, the Head of the Department of Zoology, Entomology and Fisheries Sciences, Dr. Godfrey Kawooya expressed gratitude for the enduring partnership, noting that it has significantly strengthened regional training capacity and supported the education of numerous Makerere staff and students. He added that the collaboration reinforces Makerere’s standing as one of the most collaborative universities globally and aligns closely with its research agenda.
Rector Schulev-Steindl reaffirmed BOKU’s commitment to advancing the longstanding relationship, describing it as a model of North-South academic cooperation built on mutual respect, shared knowledge and tangible impact.
Event pictorial: https://drive.google.com/drive/folders/1HkCCZf_TCPV0V7_ZR2PSVEw1zrEGhZeJ?usp=sharing
Natural Sciences
Simon Mungudit: Mak’s Best Performing Male Science Student & Rising Star in Petroleum Geoscience
Natural Sciences
Enhancing Data Quality: NutriFishPlus Research Assistants Retooled Ahead of Baseline Survey
The NutriFishPlus Project team successfully conducted a one-day capacity-building training for its research assistants, centered on the Monitoring, Evaluation, and Learning (MEL) framework and the household survey tool. Held on 13th January 2026 at Ridar Hotel Seeta in Mukono District, the training aimed to improve data collection, analysis, and learning processes to support evidence-based project implementation and impact assessment.
It was officially opened by Dr. Jackson Efitre, the Principal Investigator, who introduced the research assistants to the overall goal of the NutriFishPlus Project and the guiding principles of the International Development Research Centre (IDRC) Scaling Approach. In his remarks, Dr Efitre emphasized the importance of high-quality data in tracking progress, informing adaptive management, and scaling impact.
NutriFishPlus builds on the significant achievements of the ground-breaking NutriFish Project (2019–2023), which was funded by IDRC and the Australian Centre for International Agricultural Research (ACIAR) through the Cultivate Africa’s Future Fund Phase II (CultiAF2). Officially launched on 28th October 2025, the two-year and eight-month project seeks to scale proven innovations while deepening nutrition, livelihood, and gender outcomes within small pelagic fisheries.
The project aims to:
- Scale up the adoption of improved and sustainable fish processing technologies, including solar tent driers and raised racks, to reach new fishing communities across Uganda.
- Enhance market access and strengthen supply chain linkages for high-quality fish and fish-based products.
- Empower women and other vulnerable groups while strengthening community resilience through diversified income-generating activities.
Expected Outcomes:
NutriFishPlus is expected to deliver multiple socioeconomic and environmental benefits, including:
- Improved incomes and livelihoods for marginalized fishing communities, particularly women and youth.
- Enhanced health and nutrition outcomes through the development of diversified, market-embedded fish products.
- Establishment of sustainable and replicable fish processing and marketing models across Uganda and the wider East African region.
- Increased participation of women and youth in decision-making and benefit-sharing within the Small Pelagic Fishes (SPFs) value chain.
- Improved socioeconomic conditions and ecosystem health through participatory and scalable approaches.
Project Implementation and Partnerships
The project is implemented by the Department of Zoology, Entomology, and Fisheries Sciences at Makerere University, in partnership with Nutreal Ltd and Kati Farms Ltd under a public-private partnership. Project activities will be carried out at five landing sites across Lakes Victoria, Kyoga, and Albert.
The project team comprises:
- Dr. Jackson Efitre (Principal Investigator)
- Dr. Dorothy Nakimbugwe (Co-PI from Nutreal Ltd)
- Ms. Lovin Kobusingye (Co-PI from Kati Farms Ltd)
- Dr. Robinson Odong (Project Officer, Department of Zoology, Entomology and Fisheries Sciences, Makerere University)
- Ms. Juliet Ogubi Nafula (M&E Expert)
- Ms. Nelly Badaru (Gender Specialist)
- Mr. Eugene Swinnerstone Miheso (IT Expert)
- Mr. Maurice Ssebisubi (Kati Farms Ltd)
- Ms. Hasifa Kabejja (Communication Officer)
- Ms. Eve Akongo Ampa (Project Administrator)
Strengthening MEL and Data Quality
During the training workshop, Ms. Juliet Ogubi Nafula guided the team through the NutriFishPlus MEL framework, highlighting its objectives, indicators, results chain, and reporting requirements. Particular emphasis was placed on the role of high-quality data in facilitating project learning, adaptive management, and accountability to donors and beneficiaries.
A key highlight of the training was a hands-on orientation to the household survey tool, led by Mr. Eugene Swinnerstone Miheso. Participants were taken through the structure of the questionnaire, which covers household demographics, nutrition and dietary diversity, fish consumption, livelihoods, value addition, gender dynamics, and food security. Practical sessions enabled the research assistants to practice administering the tool, apply effective probing techniques, and address common field-level challenges.
The training underscored critical ethical considerations in household data collection, including informed consent, confidentiality, and respectful engagement with respondents. Data quality assurance measures such as accurate data recording, use of digital data collection platforms, and daily review protocols were discussed to ensure consistency and reliability.
In his closing remarks, Dr. Efitre emphasized that well-trained research assistants are central to generating credible evidence that reflects the lived realities of beneficiary households. “This training is a vital investment in strengthening our MEL system and ensuring that the evidence we generate informs learning, accountability, and improved project outcomes,” he noted.
The participants expressed gratitude for the interactive and practical nature of the training, noting that the sessions significantly enhanced their confidence and readiness for fieldwork. The training concluded with a question-and-answer session and a brief assessment to confirm participants’ understanding of the MEL framework and household survey tool.
Pre-testing of the household survey tool
Following the training, the project team conducted a pre-test of the household survey tool at Kasenyi Landing Site in Entebbe on 19th January 2026, ahead of the baseline survey. The baseline data collection will be carried out across five landing sites: Katosi (Mukono District), Kikondo (Buikwe District), Bangaladesh and Kayago (Amolatar District), and Dei (Pakwach District), spanning Lakes Victoria, Kyoga, and Albert.
Details on the project at: https://news.mak.ac.ug/2025/10/mak-conas-launches-nutrifishplus-project/
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